A mechanism for actin filament severing by malaria parasite actin depolymerizing factor 1 via a low affinity binding interface

W Wong; AI Webb; MA Olshina; G Infusini; YH Tan; E Hanssen; B Catimel; C Suarez; M Condron; F Angrisano; T Nebl; DR Kovar; J Baum

Journal article

A mechanism for actin filament severing by malaria parasite actin depolymerizing factor 1 via a low affinity binding interface

W Wong, AI Webb, MA Olshina, G Infusini, YH Tan, E Hanssen, B Catimel, C Suarez, M Condron, F Angrisano, T Nebl, DR Kovar, J Baum

Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2014

DOI: 10.1074/jbc.M113.523365

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Abstract

Actin depolymerizing factor (ADF)/cofilins are essential regulators of actin turnover in eukaryotic cells. These multifunctional proteins facilitate both stabilization and severing of filamentous (F)-actin in a concentration-dependent manner. At high concentrations ADF/cofilins bind stably to F-actin longitudinally between two adjacent actin protomers forming what is called a decorative interaction. Low densities of ADF/cofilins, in contrast, result in the optimal severing of the filament. To date, how these two contrasting modalities are achieved by the same protein remains uncertain. Here, we define the proximate amino acids between the actin filament and the malaria parasite ADF/cofilin, ..

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University of Melbourne Researchers

Andrew Webb Author

Eric Hanssen Author

Fiona Angrisano Author

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Awarded by University of Melbourne

Funding Acknowledgements

[ "The work was supported in part by National Health and Medical Research Council of Australia (NHMRC) Project Grant APP1024678 (to J. B.), Human Frontier Science Program (HFSP) Young Investigator Program Grant RGY0071/2011 (to J. B. and D. R. K.), and Australian Research Council (ARC) Discovery Project Grant DP1094497 (to B. C.), Victorian State Government Operational Infrastructure Support, and Australian Government NHMRC IRIISS.", "Supported through Early Career Fellowship APP1053801 from the NHMRC.", "Supported through NHMRC Dora Lush Scholarship APP1018002 and an Oversea Research Experience Scholarship and travel award provided by the University of Melbourne and the Australian Society of Parasitology, respectively.", "Supported by Future Fellowship FT100100112 from the Australian Research Council (ARC) and currently supported by a New Investigator Award from the Wellcome Trust 100993/Z/13/Z. To whom correspondence should be addressed: Dept. of Life Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom. E-mail: jake.baum@imperial.ac.uk." ]